pdms polydimethylsiloxane microchannel  (Cellvis Inc)

Journal: Micromachines

Article Title: Structural Reinforcement Effect of a Flexible Strain Sensor Integrated with Pneumatic Balloon Actuators for Soft Microrobot Fingers

doi: 10.3390/mi12040395

Figure Lengend Snippet: Fabrication process of liquid metal-based-sensor for use with PBAs. Mold structures preparation for PBA ( a-1 ) and sensor ( a-2 ) on separate substrates. ( b ) Spin-coating formation of three polydimethylsiloxane (PDMS) films for the PBA, a buffer area and a sensor. ( c ) Bonding of three PDMS films by vacuum ultra violet (VUV) technology. ( d ) Implementation of PDMS interconnection block. ( e ) Forming of inlets and microchannels. ( f ) Introducing of liquid metal into microchannels for a sensor. ( g ) Wiring. ( e′ ) Masking of structure except an inlet for a microchannel for a sensor. ( e″ ) Parylene C deposition into a microchannel for a sensor.

Article Snippet: Both PBA and a strain sensor use microchannel structures formed by molding polydimethylsiloxane (PDMS, Silpot 184, Dow Corning Inc., Bucharest, Romania) on a casting mold.

Techniques: Blocking Assay

Journal: Biotechnology journal

Article Title: Sacrificial Bioprinting of A Mammary Ductal Carcinoma Model

doi: 10.1002/biot.201700703

Figure Lengend Snippet: Sacrificial bioprinting of hydrogel-embedded hollow microchannels. (A) Schematics showing a typical procedure of sacrificial bioprinting process: i) Deposition of a thin layer of GelMA at the bottom of the mold; ii) bioprinting of agarose microfibers into the mold; iii) casting of the mold with GelMA prepolymer; iv) induction of gelation using photocrosslinking; v) removal of the agarose microfiber manually or using mild vacuum; vi) seeding of the mammary ductal carcinoma cells on the interior walls of the bioprinted microchannel; and vii) a variety of different shapes mimicking the mammary ducts can be generated using such a method. (B-E) Photographs showing the bioprinted agarose microfibers in the cast GelMA hydrogel, microchannels formed after removal of the agarose microfibers, the removed agarose microfibers, and perfusion of the microchannels.

Article Snippet: One relevant report took advantage of microfabricated microchannels in a polydimethylsiloxane (PDMS) chip device to form biomimetic mammary ducts with seeded human mammary epithelial cells for screening of theranostics [ 32 ].

Techniques: Generated

Journal: Biotechnology journal

Article Title: Sacrificial Bioprinting of A Mammary Ductal Carcinoma Model

doi: 10.1002/biot.201700703

Figure Lengend Snippet: Proliferation of the seeded MCF-7 cells within the bioprinted mammary duct-like microchannels. (A-F) Optical micrographs showing the proliferation of the cells over a culture period of 24 days. (G) Quantification of percentage coverage of the cells. (H) Quantification of the invasion distance of the cells into the surrounding matrix.

Article Snippet: One relevant report took advantage of microfabricated microchannels in a polydimethylsiloxane (PDMS) chip device to form biomimetic mammary ducts with seeded human mammary epithelial cells for screening of theranostics [ 32 ].

Techniques:

Journal: eLife

Article Title: Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity

doi: 10.7554/eLife.58046

Figure Lengend Snippet: Control LifeAct-GFP T cells were activated ex-vivo, labeled with Hoechst to visualize their nuclei, and added to microchannel devices. T cells crawling in the microchannels were imaged at 37°C for 2–6 hr. Brightfield and fluorescent images were acquired every 30 s. Top images, LifeAct-GFP (green) and Hoechst (DNA, blue) overlaid on the brightfield channel (gray). Bottom images, pseudocolor rendition of the LifeAct-GFP channel. This representative control LifeAct-GFP T cell shows posterior perinuclear actin polymerization while migrating through a microchannel constriction. Time is displayed as min:s.

Article Snippet: PDMS (polydimethylsiloxane) microchannel fluidic devices with constrictions were purchased from 4DCell (Montreuil, France).

Techniques:

Journal: eLife

Article Title: Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity

doi: 10.7554/eLife.58046

Figure Lengend Snippet: FMNL1 KO/LifeAct-GFP T cells were activated ex-vivo, labeled with Hoechst to visualize their nuclei, and added to microchannel devices. T cells crawling in the microchannels were imaged at 37°C for 2–6 hr. Brightfield and fluorescent images were acquired every 30 s. Top images, LifeAct-GFP (green) and Hoechst (DNA, blue) overlaid on the brightfield channel (gray). Bottom images, pseudocolor rendition of the LifeAct-GFP channel. This representative FMNL1 KO/LifeAct-GFP T cell lacks posterior perinuclear actin polymerization as its nucleus engages the microchannel constriction and is unable to migrate through the constriction. Time is displayed as min:s.

Article Snippet: PDMS (polydimethylsiloxane) microchannel fluidic devices with constrictions were purchased from 4DCell (Montreuil, France).

Techniques:

Journal: eLife

Article Title: Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity

doi: 10.7554/eLife.58046

Figure Lengend Snippet: Ex vivo activated control LifeAct-GFP and FMNL1 KO LifeAct-GFP T cells were stained with Hoechst, added to PDMS microchannels and imaged by spinning-disk confocal time-lapse microscopy. ( A ) Schematic of the PDMS microchannels with constrictions used in the experiments. ( B ) FMNL1 deficiency impairs the ability of T cells to migrate through 3 µm constrictions. Quantification of the percentage of T cell passage through 3 µm constrictions within microchannel devices. ( C ) Example images of WT LifeAct-GFP (Left panels and ) and FMNL1 KO/LifeAct-GFP (Right panels and ) T cells engaging microchannel constrictions. Top images, LifeAct-GFP (green) and Hoechst (DNA, blue) overlaid on the brightfield channel (gray). Bottom images, pseudocolor rendition of the LifeAct-GFP channel. White dashed arrows indicate cell direction, red arrows point to areas of F-actin accumulation at the back of the cell, black arrows indicate F-actin accumulation at the front of the cell. Time is min:sec, white scale bar = 10 µm. ( D ) FMNL1 promotes actin polymerization at the back of the nucleus during migration under confinement. Top, example of the image masking process to quantify F-actin distribution relative to the front and back of the nucleus. Bottom, quantification of the back-to-front ratio of F-actin distribution during unconfined and confined migration. ( E ) Paired analysis of individual T cells undergoing unconfined and confined migration. Data for cells that increase their F-actin back-to-front ratio under confinement are shown in black, data for cells that decrease the back-to-front ratio are in red. Data in B are the mean ± SEM from 3 independent experiments with a total of 83 control and 68 KO cells analyzed. Data in D are the mean ± SEM and data in E are pooled from 3 independent experiments with a total of 47 control and 35 KO cells analyzed. Statistics in B and E calculated using a two-tailed paired t-test; statistics in D calculated using One-way ANOVA with Sidak’s multiple comparisons. n.s. = not significant. Figure 7—source data 1. Data points for the graphs in are provided as an Excel spreadsheet.

Article Snippet: PDMS (polydimethylsiloxane) microchannel fluidic devices with constrictions were purchased from 4DCell (Montreuil, France).

Techniques: Ex Vivo, Control, Staining, Time-lapse Microscopy, Migration, Two Tailed Test

Journal: eLife

Article Title: Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity

doi: 10.7554/eLife.58046

Figure Lengend Snippet:

Article Snippet: PDMS (polydimethylsiloxane) microchannel fluidic devices with constrictions were purchased from 4DCell (Montreuil, France).

Techniques: Expressing, Construct, Recombinant, Plasmid Preparation, Control